Farkas, Laszlo and Farkas, Daniela and Ask, Kjetil and Moeller, Antje and Gauldie, Jack and Margetts, Peter and Inman, Mark and Kolb, Martin (2009) VEGF ameliorates pulmonary hypertension through inhibition of endothelial apoptosis in experimental lung fibrosis in rats. JOURNAL OF CLINICAL INVESTIGATION, 119 (5). pp. 1298-1311. ISSN 0021-9738, 1558-8238
Full text not available from this repository. (Request a copy)Abstract
Idiopathic pulmonary fibrosis (IPF) can lead to the development of secondary pulmonary hypertension (PH) and ultimately death. Despite this known association, the precise mechanism of disease remains unknown. Using a rat model of IPF, we explored the role of the proangiogenic and antiapoptotic growth factor VEGF in the vascular remodeling that underlies PH. In this model, adenoviral delivery of active TGF-beta 1 induces pulmonary arterial remodeling, loss of the microvasculature in fibrotic areas, and increased pulmonary arterial pressure (PAP). Immunohistochemistry and mRNA analysis revealed decreased levels of VEGF and its receptor, which were inversely correlated with PAP and endothelial cell apoptosis in both the micro- and macrovasculature. Treatment of IPF rats with adenoviral delivery of VEGF resulted in reduced endothelial apoptosis, increased vascularization, and improved PAP due to reduced remodeling but worsened PF. These data show that experimental pulmonary fibrosis (PF) leads to loss of the microvasculature through increased apoptosis and to remodeling of the pulmonary arteries, with both processes resulting in PH. As administration of VEGF ameliorated the PH in this model but concomitantly aggravated the fibrogenic process, VEGF-based therapies should be used with caution.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR-BETA; EPITHELIUM-DERIVED FACTOR; SMOOTH-MUSCLE-CELLS; GENE-TRANSFER; PIGMENT-EPITHELIUM; ARTERIAL-HYPERTENSION; DISEASE; PROLIFERATION; ANGIOGENESIS; EXPRESSION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Sep 2020 11:52 |
| Last Modified: | 16 Sep 2020 11:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29059 |
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