Salamon, Daniel and Banati, Ferenc and Koroknai, Anita and Ravasz, Mate and Szenthe, Kalman and Bathori, Zoltan and Bakos, Agnes and Niller, Hans Helmut and Wolf, Hans and Minarovits, Janos (2009) Binding of CCCTC-binding factor in vivo to the region located between Rep* and the C promoter of Epstein-Barr virus is unaffected by CpG methylation and does not correlate with Cp activity. JOURNAL OF GENERAL VIROLOGY, 90. pp. 1183-1189. ISSN 0022-1317, 1465-2099
Full text not available from this repository. (Request a copy)Abstract
In this study, the binding of the insulator protein CCCTC-binding factor (CTCF) to the region located between Rep* and the C promoter (Cp) of Epstein-Barr virus (EBV) was analysed using chromatin immunoprecipitation and in vivo footprinting. CTCF binding was found to be independent of Cp usage in cell lines corresponding to the major EBV latency types. Bisulfite sequencing and an electrophoretic mobility-shift assay (using methylated and unmethylated probes) revealed that CTCF binding was insufficient to induce local CpG demethylation in certain cell lines and was unaffected by CpG methylation in the region between Rep* and Cp. In addition, CTCF binding to the latency promoter, Op, did not correlate with Op activity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | III-TRANSCRIBED EBER-1; PROTEIN-DNA BINDING; CELL LINE C666-1; LATENT PROMOTER; RESOLUTION; GENOMES; CTCF; QP; EPIGENETICS; EXPRESSION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Sep 2020 12:06 |
| Last Modified: | 16 Sep 2020 12:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29064 |
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