Galler, K. M. and Widbiller, M. and Buchalla, W. and Eidt, A. and Hiller, K. -A. and Hoffer, P. C. and Schmalz, G. (2016) EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells. INTERNATIONAL ENDODONTIC JOURNAL, 49 (6). pp. 581-590. ISSN 0143-2885, 1365-2591
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Aim To evaluate the effect of dentine conditioning on migration, adhesion and differentiation of dental pulp stem cells. Methodology Dentine discs prepared from extracted human molars were pre-treated with EDTA (10%), NaOCl (5.25%) or H-2 O. Migration of dental pulp stem cells towards pre-treated dentine after 24 and 48 h was assessed in a modified Boyden chamber assay. Cell adhesion was evaluated indirectly by measuring cell viability. Expression of mineralization-associated genes (COL1A1, ALP, BSP, DSPP, RUNX2) in cells cultured on pre-treated dentine for 7 days was determined by RT-qPCR. Nonparametric statistical analysis was performed for cell migration and cell viability data to compare different groups and time-points (Mann-Whitney U-test, alpha = 0.05). Results Treatment of dentine with H2O or EDTA allowed for cell attachment, which was prohibited by NaOCl with statistical significance (P = 0.000). Furthermore, EDTA conditioning induced cell migration towards dentine. The expression of mineralization-associated genes was increased in dental pulp cells cultured on dentine after EDTA conditioning compared to H2O-pre-treated dentine discs. Conclusions EDTA conditioning of dentine promoted the adhesion, migration and differentiation of dental pulp stem cells towards or onto dentine. A pretreatment with EDTA as the final step of an irrigation protocol for regenerative endodontic procedures has the potential to act favourably on new tissue formation within the root canal.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MATRIX COMPONENTS; GROWTH-FACTORS; TGF-BETA; IGF-I; SOLUBILIZATION; ENDODONTICS; RELEASE; BINDING; FGF2; dental pulp stem cells; dentine; EDTA; growth factors; regenerative endodontics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Mar 2019 14:05 |
| Last Modified: | 21 Mar 2019 14:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2911 |
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