Leiherer, Andreas and Ludwig, Christine and Wagner, Ralf (2009) Influence of extended mutations of the HIV-1 transframe protein p6*on Nef-dependent viral replication and infectivity in vitro. VIROLOGY, 387 (1). pp. 200-210. ISSN 0042-6822,
Full text not available from this repository. (Request a copy)Abstract
The HIV-1 transframe protein p6* known to modulate HIV-1 protease activation has been suggested to interact with the viral pathogenicity factor Nef. However, a potential interaction site in p6* has not been mapped so far. To evaluate effects of p6* modification on viral replication in light of Nef function, clustered substitutions were introduced into the central p6* region of the infectious provirus NL4-3 and virus growth and composition of the various mutants was analyzed in different cell cultures in the presence or absence of Nef. Whereas clustered p6* substitutions did neither affect particle incorporation of Nef, nor precursor maturation or viral infectivity, a simultaneous substitution of 40 of the total 56 p6* residues significantly diminished viral infectivity and replication in a Nef-independent manner. Furthermore, this extended modification was not capable of rescuing the negative effects of a transdominant Nef mutant on particle production suggesting that the proposed target for Nef interaction in Gag-Pol is located outside the modified p6* region. In sum these data strongly argue against a functional connection of the central p6* region and Nef during viral life cycle. (C) 2009 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN-IMMUNODEFICIENCY-VIRUS; TYPE-1 P6 PROTEIN; SPACER PEPTIDE P1; AIDS VIRUS; NUCLEOTIDE-SEQUENCE; CLEAVAGE SITES; CENTRAL REGION; GAG P6; DOMAIN; ASSOCIATION; p6*; p6pol; Transframe protein; HIV-1; Frameshift; Gag-Pol; Nef; Viral infectivity; Competition assay |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Sep 2020 09:34 |
| Last Modified: | 17 Sep 2020 09:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29110 |
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