Linsel-Nitschke, Patrick and Jansen, Henning and Aherrarhou, Zouhair and Belz, Stefanie and Mayer, Bjoern and Lieb, Wolfgang and Huber, Fritz and Kremer, Werner and Kalbitzer, Hans-Robert and Erdmann, Jeanette and Schunkert, Heribert (2009) Macrophage cholesterol efflux correlates with lipoprotein subclass distribution and risk of obstructive coronary artery disease in patients undergoing coronary angiography. LIPIDS IN HEALTH AND DISEASE, 8: 14. ISSN 1476-511X,
Full text not available from this repository. (Request a copy)Abstract
Background: Studies in patients with low HDL have suggested that impaired cellular cholesterol efflux is a heritable phenotype increasing atherosclerosis risk. Less is known about the association of macrophage cholesterol efflux with lipid profiles and CAD risk in normolipidemic subjects. We have therefore measured macrophage cholesterol efflux in 142 normolipidemic subjects undergoing coronary angiography. Methods: Monocytes isolated from blood samples of patients scheduled for cardiac catheterization were differentiated into macrophages over seven days. Isotopic cholesterol efflux to exogenously added apolipoprotein A-I and HDL2 was measured. Quantitative cholesterol efflux from macrophages was correlated with lipoprotein subclass distribution in plasma from the same individuals measured by NMR-spectroscopy of lipids and with the extent of coronary artery disease seen on coronary angiography. Results: Macrophage cholesterol efflux was positively correlated with particle concentration of smaller HDL and LDL particles but not with total plasma concentrations of HDL or LDL-cholesterol. We observed an inverse relationship between macrophage cholesterol efflux and the concntration of larger and triglyceride rich particles (VLDL, chylomicrons). Subjects with significant stenosis on coronary angiography had lower cholesterol efflux from macrophages compared to individuals without significant stenosis (adjusted p = 0.02). Conclusion: Macrophage cholesterol efflux is inversely correlated with lipoprotein particle size and risk of CAD.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CASSETTE TRANSPORTER 1; TANGIER-DISEASE; CELLULAR CHOLESTEROL; HEPATIC ABCA1; LOW HDL; HYPOALPHALIPOPROTEINEMIA; INACTIVATION; DEFICIENCY; MUTATIONS; MICE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Sep 2020 06:44 |
| Last Modified: | 18 Sep 2020 06:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29130 |
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