Lorenz, Baerbel and Mey, Ingo and Steltenkamp, Siegfried and Fine, Tamir and Rommel, Christina and Mueller, Martin Michael and Maiwald, Alexander and Wegener, Joachim and Steinem, Claudia and Janshoff, Andreas (2009) Elasticity Mapping of Pore-Suspending Native Cell Membranes. SMALL, 5 (7). pp. 832-838. ISSN 1613-6810, 1613-6829
Full text not available from this repository. (Request a copy)Abstract
The mechanics of cellular membranes are governed by a non-equilibrium composite framework consisting of the semiflexible filamentous cytoskeleton and extracellular matrix proteins linked to the lipid bilayer. While elasticity information of plasma membranes has mainly been obtained from whole cell analysis, techniques that allow addressing local mechanical properties of cell membranes are desirable to learn how their lipid and protein composition is reflected in the elastic behavior on local length scales. Introduced here is an approach based on basolateral membranes of polar epithelial Madin-Darby canine kidney (MDCK) H cells, prepared on a highly ordered porous substrate that allows elastic mapping on a submicrometer-length scale. A strong correlation between the density of actin filaments and the measured membrane elasticity is found. Spatially resolved indentation experiments carried out with atomic force and fluorescence microscope permit relation of the supramolecular structure to the elasticity of cellular membranes. It is shown that the elastic response of the pore spanning cell membranes is governed by local bending modules rather than lateral tension.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ATOMIC-FORCE MICROSCOPY; LIPID-BILAYERS; FLUID MEMBRANES; MECHANICS; TENSION; BIOMEMBRANES; SPECTROSCOPY; DEFORMATION; ALDOSTERONE; TENSEGRITY; atomic force microscopy; bending rigidity; epithelial cells; indentation; lateral tension |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Sep 2020 06:46 |
| Last Modified: | 18 Sep 2020 06:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29131 |
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