Riechers, Alexander and Schmidt, Florian and Stadlbauer, Stefan and Koenig, Burkhard (2009) Detection of Protein Phosphorylation on SDS-PAGE Using Probes with a Phosphate-Sensitive Emission Response. BIOCONJUGATE CHEMISTRY, 20 (4). pp. 804-807. ISSN 1043-1802,
Full text not available from this repository. (Request a copy)Abstract
Fluorescent probes for the detection of protein phosphorylation on SDS-PAGE are presented. The probes were designed using a dinuclear metal-chelate phosphate recognition unit and an environmentally sensitive fluorophore. The specificity of the probes is determined by their binding site selectivity toward phosphate ions and the emission response induced by the change in the electrostatic environment of the fluorophore upon binding to a phosphorylated amino acid residue. The staining is fully reversible due to the noncovalent binding of the probe. Gel bands with less than 100 ng of phosphorylated alpha-casein are detectable with the new probes on a normal UV-table without specialized equipment like a laser-based gel scanner or a cooled camera detector.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MASS-SPECTROMETRY; TYROSINE PHOSPHORYLATION; GEL-ELECTROPHORESIS; AQUEOUS-SOLUTION; RECOGNITION; COMPLEMENT; RECEPTORS; PEPTIDE; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Sep 2020 08:41 |
| Last Modified: | 18 Sep 2020 08:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29144 |
Actions (login required)
 |
View Item |
