Blume, Annegret and Torner, Luz and Liu, Ying and Subburaju, Sivan and Aguilera, Greti and Neumann, Inga D. (2009) Prolactin Activates Mitogen-Activated Protein Kinase Signaling and Corticotropin Releasing Hormone Transcription in Rat Hypothalamic Neurons. ENDOCRINOLOGY, 150 (4). pp. 1841-1849. ISSN 0013-7227, 1945-7170
Full text not available from this repository. (Request a copy)Abstract
Prolactin (PRL) modulates maternal behavior and mediates hypothalamic pituitary adrenal axis inhibition during lactation via PRL receptors in the brain. To identify mechanisms mediating these effects, we examined the effects of PRL on signaling and CRH transcription in hypothalamic neurons in vivo and in vitro. Western blot of hypothalamic proteins from rats receiving intracerebro-ventricular PRL injection revealed increases in phosphorylation of the MAPK and ERK. Double-staining immunohistochemistry demonstrated phosphorylated ERK localization in parvocellular CRH neurons as well as magnocellular vasopressin and oxytocin neurons of the hypothalamic paraventricular (PVN) and supraoptic nuclei. PRL also induced ERK phosphorylation in vitro in the hypothalamic cell line, 4B, which expresses PRL receptors, and in primary hypothalamic neuronal cultures. Using reporter gene assays in 4B cells, or quantitative RT-PCR for primary transcript in hypothalamic cell cultures, PRL potentiated forskolin-stimulated CRH transcription through activation of the ERK/MAPK pathway. The effect of PRL in hypothalamic cell cultures was unaffected by tetrodotoxin, suggesting a direct effect on CRH neurons. The data show that PRL activates the ERK/MAPK pathway and facilitates CRH transcription in CRH neurons, suggesting that the inhibitory effect of PRL on hypothalamo-pituitary-adrenal axis activity reported in vivo is indirect and probably mediated through modulation of afferent pathways to the PVN. In addition, the prominent stimulatory action of PRL on the ERK/MAPK pathway in the hypothalamic PVN and supraoptic nucleus is likely to mediate neuroplasticity of the neuroendocrine system during lactation. (Endocrinology 150: 1841-1849, 2009)
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RECEPTOR MESSENGER-RNA; TUBEROINFUNDIBULAR DOPAMINERGIC-NEURONS; NEUROENDOCRINE STRESS RESPONSES; NB2 LYMPHOMA-CELLS; GENE-EXPRESSION; TYROSINE-HYDROXYLASE; MATERNAL-BEHAVIOR; LACTATING RATS; MAP KINASE; PARAVENTRICULAR NUCLEUS; |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Tierphysiologie/Neurobiologie (Prof. Dr. Inga Neumann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Sep 2020 09:02 |
| Last Modified: | 18 Sep 2020 09:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29157 |
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