Okuda, Satohiro and Tsutsui, Hiroki and Shiina, Keiko and Sprunck, Stefanie and Takeuchi, Hidenori and Yui, Ryoko and Kasahara, Ryushiro D. and Hamamura, Yuki and Mizukami, Akane and Susaki, Daichi and Kawano, Nao and Sakakibara, Takashi and Namiki, Shoko and Itoh, Kie and Otsuka, Kurataka and Matsuzaki, Motomichi and Nozaki, Hisayoshi and Kuroiwa, Tsuneyoshi and Nakano, Akihiko and Kanaoka, Masahiro M. and Dresselhaus, Thomas and Sasaki, Narie and Higashiyama, Tetsuya (2009) Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells. NATURE, 458 (7236). 357-U122. ISSN 0028-0836, 1476-4687
Full text not available from this repository. (Request a copy)Abstract
For more than 140 years, pollen tube guidance in flowering plants has been thought to be mediated by chemoattractants derived from target ovules(1). However, there has been no convincing evidence of any particular molecule being the true attractant that actually controls the navigation of pollen tubes towards ovules. Emerging data indicate that two synergid cells on the side of the egg cell emit a diffusible, species-specific signal to attract the pollen tube at the last step of pollen tube guidance(1-3). Here we report that secreted, cysteine-rich polypeptides (CRPs) in a subgroup of defensin-like proteins are attractants derived from the synergid cells. We isolated synergid cells of Torenia fournieri, a unique plant with a protruding embryo sac, to identify transcripts encoding secreted proteins as candidate molecules for the chemoattractant(s). We found two CRPs, abundantly and predominantly expressed in the synergid cell, which are secreted to the surface of the egg apparatus. Moreover, they showed activity in vitro to attract competent pollen tubes of their own species and were named as LUREs. Injection of morpholino antisense oligomers against the LUREs impaired pollen tube attraction, supporting the finding that LUREs are the attractants derived from the synergid cells of T. fournieri.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TORENIA-FOURNIERI; FEMALE GAMETOPHYTE; ANTIMICROBIAL PEPTIDES; DOUBLE FERTILIZATION; EMBRYO SAC; IN-VITRO; GUIDANCE; ARABIDOPSIS; GENES; PROTEIN; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Sep 2020 07:16 |
| Last Modified: | 21 Sep 2020 07:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29268 |
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