Goertz, O. and Kirschner, M. H. and Lilienfein, H. and Babilas, P. and Steinau, H. U. and Andree, C. and Daigeler, A. and Stachon, A. and Homann, H. and Langer, S. (2009) EFFECTS OF MALEIMIDE-POLYETHYLENE GLYCOL-MODIFIED HUMAN HEMOGLOBIN (MP4) ON TISSUE NECROSIS IN SKH1-HR HAIRLESS MICE. EUROPEAN JOURNAL OF MEDICAL RESEARCH, 14 (3). pp. 123-129. ISSN 0949-2321,
Full text not available from this repository. (Request a copy)Abstract
Objective: Tissue hypoxia after blood loss, replantation and flap reperfusion remains a challenging task in surgery. Normovolemic hemodilution improves hemorheologic properties without increasing oxygen carrying capacity. Red blood cell transfusion is the current standard of treatment with its attendant risks. The aim of this study was to investigate the potential of the chemically, modified hemoglobin, MP4, to reduce skin flap necrosis and its effect on selected blood markers and kidneys. Materials and Methods: Tissue ischemia was induced in the car of hairless mice (n=26). Hemodilution was performed by replacing one third of blood volume with the similar amount of MP4, dextran, or blood. The extent of non-perfused tissue was assessed by intravital fluorescent microscopy. Results: Of all groups, MP4 showed the smallest area of no perfusion (in percentage of the ear +/- SEM: 16.3% +/- 2.4), the control group the largest (22.4% +/- 3.5). Leukocytes showed a significant increase in the MP4 and dextran group (from 8.7 to 13.6 respectively 15.4*10(9)/1). On histology no changes of the kidneys could be observed. Conclusion: MP4 causes an increase of leukocytes, improves the oxygen supply of the tissue and shows no evidence of renal impairment.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SKIN FLAP FAILURE; OXYGEN-TRANSPORT; BLOOD-FLOW; MODEL; MICROCIRCULATION; HEMODILUTION; MICROSCOPY; REDUCTION; CARRIERS; SAFETY; MP4; hairless mice; intravital fluorescent microscopy; oxygen carrier; free hemoglobin; intracardiac access; hemodilution |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Sep 2020 08:06 |
| Last Modified: | 21 Sep 2020 08:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29272 |
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