Hasselblatt, Martin and Mertsch, Sonja and Koos, Bjoern and Riesmeier, Barbara and Stegemann, Heike and Jeibmann, Astrid and Tomm, Manuel and Schmitz, Nicole and Wrede, Brigitte and Wolff, Johannes E. and Zheng, Wei and Paulus, Werner (2009) TWIST-1 Is Overexpressed in Neoplastic Choroid Plexus Epithelial Cells and Promotes Proliferation and Invasion. CANCER RESEARCH, 69 (6). pp. 2219-2223. ISSN 0008-5472,
Full text not available from this repository. (Request a copy)Abstract
The pathogenesis of choroid plexus papillomas, intraventricular papillary neoplasms most often occurring sporadically in children and young adults, remains poorly understood. To identify pathways operative in the development of choroid plexus papillomas, gene expression profiles obtained from laser-microdissected human choroid plexus papilloma cells (n = 7) were compared with that of normal choroid plexus epithelial cells laser microdissected from autopsy tissue (n = 8). On DNA microarray data analysis, 53 probe sets were differentially expressed in choroid plexus papilloma tumor cells (>7-fold). Up-regulation of TWIST-1, WIF1, TRPM3, BCLAF-1, and AJAP1, as well as down-regulation of IL6ST was confirmed using quantitative reverse transcription-PCR. Knockdown of Twist1 gene expression in the rat choroid plexus epithelial cell line Z310 significantly reduced proliferation as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell invasion in a Matrigel assay, whereas cell migration was not affected. Screening for expressional changes of cancer-related genes upon Twist1 knockdown revealed up-regulation of Cdkn1a, Cflar, and Serpinb2 and down-regulation of Figf. To conclude, using gene expression profiling, several genes differentially expressed in human choroid plexus papillomas could be identified. Among those, TWIST1 is highly expressed in choroid plexus papillomas and promotes proliferation and invasion. [Cancer Res 2009;69(6):2219-23]
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HEPATOCELLULAR-CARCINOMA; NERVOUS-SYSTEM; TUMORS; METASTASIS; EXPRESSION; MUTATIONS; CANCER; IDENTIFICATION; PROGRESSION; EPENDYMOMA; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Pädiatrische Hämatologie, Onkologie und Stammzelltransplantation |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Sep 2020 08:08 |
| Last Modified: | 21 Sep 2020 08:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29274 |
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