Clemente-Blanco, Andres and Mayan-Santos, Maria and Schneider, David A. and Machin, Felix and Jarmuz, Adam and Tschochner, Herbert and Aragon, Luis (2009) Cdc14 inhibits transcription by RNA polymerase I during anaphase. NATURE, 458 (7235). 219-U8. ISSN 0028-0836, 1476-4687
Full text not available from this repository. (Request a copy)Abstract
Chromosome condensation and the global repression of gene transcription(1) are features of mitosis inmost eukaryotes. The logic behind this phenomenon is that chromosome condensation prevents the activity of RNA polymerases. In budding yeast, however, transcription was proposed to be continuous during mitosis(2). Here we show that Cdc14, a protein phosphatase required for nucleolar segregation(3) and mitotic exit(4), inhibits transcription of yeast ribosomal genes (rDNA) during anaphase. The phosphatase activity of Cdc14 is required for RNA polymerase I (Pol I) inhibition in vitro and in vivo. Moreover Cdc14-dependent inhibition involves nucleolar exclusion of Pol I subunits. We demonstrate that transcription inhibition is necessary for complete chromosome disjunction, because ribosomal RNA (rRNA) transcripts block condensin binding to rDNA, and show that bypassing the role of Cdc14 in nucleolar segregation requires in vivo degradation of nascent transcripts. Our results show that transcription interferes with chromosome condensation, not the reverse. We conclude that budding yeast, like most eukaryotes, inhibit Pol I transcription before segregation as a prerequisite for chromosome condensation and faithful genome separation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; BUDDING YEAST; CHROMOSOME CONDENSATION; NUCLEOLAR SEGREGATION; RIBOSOMAL DNA; MITOTIC EXIT; CELL-CYCLE; RDNA; PHOSPHATASE; MITOSIS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Sep 2020 08:19 |
| Last Modified: | 21 Sep 2020 08:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29279 |
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