Bauer, Jessica and Huy, Christian and Brenmoehl, Julia and Obermeier, Florian and Bock, Juergen (2009) Matrix metalloproteinase-1 expression induced by IL-1 beta requires acid sphingomyelinase. FEBS LETTERS, 583 (5). pp. 915-920. ISSN 1873-3468,
Full text not available from this repository. (Request a copy)Abstract
Matrix metalloproteinase-1 (MMP-1) is increased in inflammatory conditions leading to destruction of extracellular matrix. Many inflammatory stimuli activate sphingomyelinases (SMases), which generate ceramide. We aimed to de. ne the relevance and type of SMase responsible for the regulation of MMP-1. Acid sphingomyelinase (ASM)-deficient human fibroblasts failed to phosphorylate extracellular signal-regulated kinase (ERK), or upregulate MMP-1 mRNA and protein expression upon stimulation with interleukin-1 beta (IL-1 beta), whereas phosphorylation of p38 mitogen-activated protein kinase and IL-8 production remained unaffected. Transfection of ASM restored MMP-1 production. Addition of exogenous SMase was sufficient to restore activation of ERK and increase MMP-1 mRNA. Inhibition of ASM with imipramine completely abrogated MMP-1 induction. The results suggest that IL-1 beta-induced expression of MMP-1 is dependent on ASM. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NF-KAPPA-B; INFLAMMATORY-BOWEL-DISEASE; PROTEIN-KINASE PATHWAYS; COLLAGENASE MMP-1; INDUCED APOPTOSIS; BINDING-PROTEIN; TERMINAL KINASE; CERAMIDE; ACTIVATION; FIBROBLAST; Extracellular matrix degradation; Collagen breakdown; Ceramide; Inflammation; Lipid metabolism; Imipramine |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Sep 2020 08:34 |
| Last Modified: | 21 Sep 2020 08:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29288 |
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