Eckhardt, Alexander and Harorli, Tolga and Limtanyakul, Jirakant and Hiller, Karl-Anton and Bosl, Claudia and Bolay, Carola and Reichl, Franz-Xaver and Schmalz, Gottfried and Schweikl, Helmut (2009) Inhibition of cytokine and surface antigen expression in LPS-stimulated murine macrophages by triethylene glycol dimethacrylate. BIOMATERIALS, 30 (9). pp. 1665-1674. ISSN 0142-9612, 1878-5905
Full text not available from this repository. (Request a copy)Abstract
Dental resin monomers like triethylene glycol dimethacrylate (TEGDMA) cause a shift in the cellular redox balance which influences redox-sensitive signaling pathways. The immediate response of the innate immune system to inflammatory challenges is controlled by related pathways. Therefore, the influence of TEGDMA on the expression of the pro- and anti-inflammatory cytokines TNF-alpha, IL-6, and IL-10 and surface antigens (CD14, CD40, CD80, CD86, CD54, MHC class I and II) was analyzed in RAW264.7 macrophages. No significant change in cytokine production or surface antigen expression was detected after the macrophages were treated with increasing TEGDMA concentrations for 6, 24, and 48 h. However, co-stimulation with the bacterial endotoxin lipopolysaccharide (LPS) and TEGDMA resulted in a concentration-dependent inhibition of LPS-induced release of TNF-alpha, IL-6, and IL-10 by about 90% as detected by ELISA. Flow-cytometric analyses indicated an LPS-stimulated expression of all surface antigens. The LPS-induced expression of CD14 was inhibited by high TEGDMA concentrations. CD40 and CD80 expressions were down-regulated by TEGDMA in LPS-stimulated cells, and CD86 as well as MHC class I expression was inhibited to a lesser extent. The LPS-stimulated expression of CD54 (ICAM-1) was increased about twofold by increasing TEGDMA concentrations after a 24 and 48 h exposure. Thus, the ability of macrophages to induce an appropriate immune response is inhibited by TEGDMA which reduces cytokine production and expression of surface antigens. (C) 2008 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NF-KAPPA-B; ICAM-1 GENE-TRANSCRIPTION; TUMOR-NECROSIS-FACTOR; ADHESION MOLECULE-1; BINDING-PROTEIN; DENDRITIC CELLS; INNATE IMMUNITY; UP-REGULATION; CROSS-TALK; ACTIVATION; Dental resin; TEGDMA; Lipopolysaccharide; Cytokine; Surface antigen |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Prof. Dr. rer. nat. Helmut Schweikl Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Sep 2020 09:50 |
| Last Modified: | 21 Sep 2020 09:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29310 |
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