Walz, Anke and Odenbreit, Stefan and Stuehler, Kai and Wattenberg, Andreas and Meyer, Helmut E. and Mahdavi, Jafar and Boren, Thomas and Ruhl, Stefan (2009) Identification of glycoprotein receptors within the human salivary proteome for the lectin-like BabA and SabA adhesins of Helicobacter pylori by fluorescence-based 2-D bacterial overlay. PROTEOMICS, 9 (6). pp. 1582-1592. ISSN 1615-9853, 1615-9861
Full text not available from this repository. (Request a copy)Abstract
Because gastric infection by Helicobacter pylori takes place via the oral route, possible interactions of this bacterium with human salivary proteins could occur. By using modified 1-and 2-D bacterial overlay, binding of H. pylori adhesins BabA and SabA to the whole range of salivary proteins was explored. Bound salivary receptor molecules were identified by MALDI-MS and by comparison to previously established proteome maps of whole and glandular salivas. By use of adhesin-deficient mutants, binding of H. pylori to MUC7 and gp-340 could be linked to the SabA and BabA adhesins, respectively, whereas binding to MUC5B was associated with both adhesins. Binding of H. pylori to the proline-rich glycoprotein was newly detected and assigned to BabA adhesin whereas the SabA adhesin was found to mediate binding to newly detected receptor molecules, including carbonic anhydrase VI, secretory component, heavy chain of secretory IgA1, parotid secretory protein and zinc-alpha(2)-glycoprotein. Some of these salivary glycoproteins are known to act as scavenger molecules or are involved in innate immunity whereas others might come to modify the pathogenetic properties of this organism. In general, this 2-D bacterial overlay technique represents a useful supplement in adhesion studies of bacteria with complex protein mixtures.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BLOOD-GROUP ANTIGENS; POLYACRYLAMIDE GELS; CARBONIC-ANHYDRASE; ORAL ACTINOMYCES; BINDING; MUCIN; INFECTION; ELECTROPHORESIS; INFLAMMATION; AGGLUTININ; Adhesins; Helicobacter pylori; Proteome map; Saliva |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Oct 2020 08:23 |
| Last Modified: | 05 Oct 2020 08:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29440 |
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