Yoshii, Taishi and Wuelbeck, Corinna and Sehadova, Hana and Veleri, Shobi and Bichler, Dominik and Stanewsky, Ralf and Helfrich-Foerster, Charlotte (2009) The Neuropeptide Pigment-Dispersing Factor Adjusts Period and Phase of Drosophila's Clock. JOURNAL OF NEUROSCIENCE, 29 (8). pp. 2597-2610. ISSN 0270-6474,
Full text not available from this repository. (Request a copy)Abstract
The neuropeptide pigment-dispersing factor (PDF) is a key transmitter in the circadian clock of Drosophila melanogaster. PDF is necessary for robust activity rhythms and is thought to couple the circadian oscillations of the clock neurons. However, little is known about the action of PDF on individual clock neurons. Here, we combined the period-luciferase reporter system with immunolabeling of clock proteins in wild-type and Pdf(01) mutants to dissect the effects of PDF on specific subgroups of clock neurons. Additionally, PDF levels were elevated to higher than normal levels using specific neural mutants, and a correlation analysis of locomotor activity and clock protein staining served to determine the periods of specific clock cells. We found that PDF has multiple effects on the clock neurons: In some groups of clock neurons, PDF was required for maintaining the oscillations of individual cells, and in others, PDF was required for synchronous cycling of the individual members. Other clock neurons cycled with high amplitude in absence of PDF, but PDF affected their intrinsic clock speed. Sometimes PDF shortened and sometimes PDF lengthened period. Our observations indicate that PDF is crucial for adjusting cycling amplitude, period, and phase of the different players in the circadian clock. Under natural conditions PDF may be required for adapting Drosophila's clock to varying photoperiods. Indeed, we show here that Pdf(01) mutants are not able to adapt their activity to long photoperiods in a wild-type manner.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CIRCADIAN CLOCK; SUPRACHIASMATIC NUCLEUS; PACEMAKER NEURONS; LATERAL NEURONS; FUNCTIONAL-ANALYSIS; DORSAL NEURONS; COMPOUND EYES; PDF NEURONS; GENE; RHYTHMS; pigment-dispersing factor; period; timeless; internal desynchronization; circadian rhythms; neuropeptide |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Alumni or Retired > Organisation der inneren Uhr auf neuronaler Ebene (Prof. Dr. Charlotte Förster) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Oct 2020 09:09 |
| Last Modified: | 05 Oct 2020 09:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29458 |
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