Schaeffler, Andreas and Gross, Philipp and Buettner, Roland and Bollheimer, Cornelius and Buechler, Christa and Neumeier, Markus and Kopp, Andrea and Schoelmerich, Juergen and Falk, Werner (2009) Fatty acid-induced induction of Toll-like receptor-4/nuclear factor-kappa B pathway in adipocytes links nutritional signalling with innate immunity. IMMUNOLOGY, 126 (2). pp. 233-245. ISSN 0019-2805, 1365-2567
Full text not available from this repository. (Request a copy)Abstract
To study the effects of fatty acids and the involvement of the Toll-like receptor-4/nuclear factor-kappa B (TLR-4/NF-kappa B) pathway with respect to the secretion of adipokines from adipocytes 3T3-L1 adipocytes were stimulated with increasing doses of fatty acids. The secretion of adiponectin, resistin and monocyte chemoattractant protein-1 (MCP-1) was measured by enzyme-linked immunosorbent assay. The NF-kappa B p65 nuclear translocation and TLR-4 expression were investigated by Western blot. The effects mediated by NF-kappa B were tested using a specific NF-kappa B-inhibitor and TLR-4-induced effects were analysed with a neutralizing TLR-4 antibody. Binding of C-14-labelled fatty acids to TLR-4/MD-2 was investigated using a FLAG-tagged extracellular part of TLR-4 fused to full-length MD-2 via a linker (lipopolysaccharide-Trap). The messenger RNA (mRNA) expression of adipokines in abdominal adipose tissue of rats fed a standard chow or a high-fat diet was investigated by reverse transcription-polymerase chain reaction. The TLR-4 is induced during adipocyte differentiation and its expression is enhanced following fatty acid stimulation. The stimulatory effects of stearic and palmitic acids on MCP-1 secretion and of palmitoleic acid on resistin secretion are mediated via NF-kappa B. The stimulatory effects of stearic, palmitic and palmitoleic acids on resistin secretion and the stimulatory effect of stearic acid on MCP-1 secretion are mediated via TLR-4. Fatty acid-mediated effects are caused by an endogenous ligand because fatty acids were shown not to bind directly to TLR-4/MD-2. Adipose tissue mRNA expression and serum levels of adipokines did not differ in rats fed a high-fat diet. These data provide a new molecular mechanism by which fatty acids can link nutrition with innate immunity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MONOCYTE CHEMOATTRACTANT PROTEIN-1; CONJUGATED LINOLEIC-ACID; ADIPOSE CELL LINE; INSULIN-RESISTANCE; METABOLIC SYNDROME; GENE-EXPRESSION; INFLAMMATORY CHANGES; RECEPTOR EXPRESSION; ADIPONECTIN; OBESITY; adipocyte; adipokines; fatty acids; innate immunity; metabolism; Toll-like receptor-4 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Oct 2020 05:29 |
| Last Modified: | 07 Oct 2020 05:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29518 |
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