Hoecherl, Klaus and Schmidt, Christoph and Bucher, Michael (2009) COX-2 inhibition attenuates endotoxin-induced downregulation of organic anion transporters in the rat renal cortex. KIDNEY INTERNATIONAL, 75 (4). pp. 373-380. ISSN 0085-2538,
Full text not available from this repository. (Request a copy)Abstract
Renal excretion of organic anions such as para-aminohippurate is reduced during severe sepsis and following ischemia/reperfusion injury. In order to better define the pathophysiology of sepsis-associated renal tubular dysfunction we measured the effect of lipopolysaccharide on renocortical organic anion transporter (OAT) expression in the rat. Prostaglandin E2 (PGE(2)) downregulates OATs in vitro, therefore, we also evaluated the effect of the cyclooxygenase (COX)-2 inhibitor parecoxib on this process. Endotoxemia caused a time- and dose-dependent decrease of OAT1 and OAT3 expression that paralleled increased renocortical COX-2 expression and PGE2 formation. Pretreatment with parecoxib decreased endotoxin-stimulated PGE2 formation. Parecoxib attenuated OAT1 and OAT3 gene repression in the rat kidney following endotoxin treatment and during ischemia/reperfusion-induced acute renal injury. COX-2 inhibition improved the creatinine clearance in lipopolysaccharide-treated rats but not after ischemia/reperfusion-induced acute renal injury. The decreased clearance of para-aminohippurate in rats following endotoxin- or ischemia/reperfusion-induced renal injury was improved by parecoxib. Our findings show that COX-2 derived prostanoids downregulate OATs during lipopolysaccharide-induced acute renal injury.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BILATERAL URETERAL OBSTRUCTION; ISCHEMIA-REPERFUSION INJURY; ISCHEMIA/REPERFUSION INJURY; CYCLOOXYGENASE-2 EXPRESSION; RENIN EXPRESSION; DRUG TRANSPORT; CORTICAL OAT1; UP-REGULATION; IN-VIVO; FAILURE; sepsis; cyclooxygenase; prostaglandins; ischemia; inflammation |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Biology, Preclinical Medicine > Institut für Physiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Oct 2020 06:08 |
| Last Modified: | 07 Oct 2020 06:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29533 |
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