Antoine, Marianne and Tag, Carmen G. and Gressner, Axel M. and c, Claus and Kiefer, Paul (2009) Expression of E-selectin ligand-1 (CFR/ESL-1) on hepatic stellate cells: Implications for leukocyte extravasation and liver metastasis. ONCOLOGY REPORTS, 21 (2). pp. 357-362. ISSN 1021-335X, 1791-2431
Full text not available from this repository. (Request a copy)Abstract
Leukocytes and tumor cells use E-selectin binding ligands to attach to activated endothelial cells expressing E-selectin during inflammation or metastasis. The cysteine-rich fibroblast growth factor receptor (CFR) represents the main E-selectin ligand (ESL-1) on granulocytes and its expression is exclusively modified by alpha(1,3)-fucosyltransferases IV or VII (FucT4 and FucT7). Hepatic stellate cells (HSC) are pericytes of liver sinusoidal endothelial cells. The activation of HSC and transdifferentiation into a myofibroblastic phenotype is involved in the repair of liver tissue injury, liver regeneration and angiogenesis of liver metastases. In the present study, we demonstrated that HSC expressed CFR together with FucT7 and exhibited a functional E-selectin binding activity on their cell surface. Since HSC appear to be oxygen-sensing cells, the expression of E-selectin binding activity was analyzed in HSC under a hypoxic atmosphere. While the expression of the glycoprotein CFR was unaffected by hypoxia, the cell-associated E-selectin binding activity decreased. However, under the same conditions, mRNA expression of the modifying enzyme FucT7 increased. The loss of E-selectin binding activity, therefore, appears to be neither the result of a reduced expression of the modifying transferase nor the expression of the backbone glycoprotein. After the transient transfection of HSC with CFR cDNA, the E-selectin binding activity (ESL-1) was efficiently released into the supernatant. Therefore, we hypothesize that under hypoxia, ESL-1 is shed from activated HSC. Our findings provide a novel perspective on the function of HSC in liver metastasis and inflammatory liver diseases.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLON-CARCINOMA CELLS; IDENTIFICATION; ESL-1; E-selectin; E-selectin ligand-1; hepatic stellate cells |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Oct 2020 07:07 |
| Last Modified: | 07 Oct 2020 07:07 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29548 |
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