Dirscherl, Georg and Schwab, Michael and Seufert, Wolfgang and Koenig, Burkhard (2009) Enhancing the separation of phosphorylated proteins in gel electrophoresis with dinuclear bispyridylmethylamine-tyrosine-acrylamide complexes. INORGANICA CHIMICA ACTA, 362 (2). pp. 537-542. ISSN 0020-1693, 1873-3255
Full text not available from this repository. (Request a copy)Abstract
Bispyridylmethylamine-tyrosine-acrylamide ligands were prepared from protected tyrosine and dipyridylmethylamine by a twofold Mannich reaction and converted into acryl amides. Their manganese complexes were used as gel additives to increase the specific phosphate affinity in SDS-PAGE protein separations. The modified gel showed a distinct mobility shift of phosphorylated alpha-casein in comparison to the dephosphorylated protein. (C) 2008 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MASS-SPECTROMETRY; KINASE ACTIVITY; LIVING CELLS; PROBES; RECOGNITION; REPORTERS; Coordination compounds; Zinc(II) complexes; Manganese(II) complexes; Phosphoproteins; Acryl amides; SDS gel electrophoresis |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Cell Cycle Control > Prof. Dr. Wolfgang Seufert Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Oct 2020 08:56 |
| Last Modified: | 07 Oct 2020 08:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29568 |
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