Tarantola, Marco and Schneider, David and Sunnick, Eva and Adam, Holger and Pierrat, Sebastien and Rosman, Christina and Breus, Vladimir and Soennichsen, Carsten and Basche, Thomas and Wegener, Joachim and Janshoff, Andreas (2009) Cytotoxicity of Metal and Semiconductor Nanoparticles Indicated by Cellular Micromotility. ACS NANO, 3 (1). pp. 213-222. ISSN 1936-0851, 1936-086X
Full text not available from this repository. (Request a copy)Abstract
In the growing field of nanotechnology, there is an urgent need to sensitively determine the toxicity of nanoparticles since many technical and medical applications are based on controlled exposure to particles, that is, as contrast agents or for drug delivery. Before the in vivo implementation, in vitro cell experiments are required to achieve a detailed knowledge of toxicity and biodegradation as a function of the nanoparticles' physical and chemical properties. In this study, we show that the micromotility of animal cells as monitored by electrical cell-substrate impedance analysis (ECIS) is highly suitable to quantify in vitro cytotoxicity of semiconductor quantum dots and gold nanorods. The method is validated by conventional cytotoxicity testing and accompanied by fluorescence and dark-field microscopy to visualize changes in the cytoskeleton integrity and to determine the location of the particles within the cell.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GOLD NANOPARTICLES; QUANTUM DOTS; IMPEDANCE SPECTROSCOPY; MAMMALIAN-CELLS; TISSUE-CULTURE; MDCK CELLS; REAL-TIME; IN-VIVO; NANORODS; TOXICITY; epithelial cells; ECIS; micromotion; transepithelial resistance (TER); cytotoxicity; gold nanorods; multishell quantum dots |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Oct 2020 10:48 |
| Last Modified: | 07 Oct 2020 10:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29590 |
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