Staudenmaier, R. and Froelich, K. and Birner, M. and Kindermann, J. and Nguyen The Hoang, and Pueschel, R. C. and Mandlik, V. (2009) Optimization of Platelet Isolation and Extraction of Autogenous TGF-beta in Cartilage Tissue Engineering. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, 37 (6). pp. 265-272. ISSN 1073-1199, 1532-4184
Full text not available from this repository. (Request a copy)Abstract
Platelets are enriched with Transforming Growth Factor-beta (TGF-beta). However, information is limited concerning TGF-beta's effects at the molecular level. Nevertheless, it has been demonstrated that TGF-beta activates cell proliferation and its postitive influence on cartilage formation has been proven within the field of Tissue Engineering (TE). As Platelet Rich Plasma (PRP) contains TGF-beta, it was the purpose of this study to optimize PRP-isolation for further TGF-beta extraction. Red blood cell count (RBC) was separated from whole blood by centrifugation. From the supernatant PRP and platelet poor plasnia (PPP) layer, the latter supematant was re-centrifuged to extract PRP. Various experimental series were run to investigate influences concerning anticoagulating altematives, different amounts of buffer, various centrifugal forces, or substituting centrifugation for sedimentation. TGF-beta levels were determined using ELISA. The technique of platelet-/TGF-beta-extraction described here proves to be more effective than other methods, is easily repeatable and nottime-consuming, which predisposes it for TE requirements.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR-BETA; RICH PLASMA; MAXILLOFACIAL SURGERY; ARTICULAR-CARTILAGE; FIBRIN GLUE; SYSTEM; KIT; GEL; autologous platelets; centrifugation; TGF-beta; cartilage; tissue engineering |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Hals-Nasen-Ohren-Heilkunde Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Oct 2020 05:12 |
| Last Modified: | 12 Oct 2020 05:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29642 |
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