Rivera, Francisco J. and Siebzehnrubl, Florian A. and Kandasamy, Mahesh and Couillard-Despres, Sebastien and Caioni, Massimiliano and Poehler, Anne-Maria and Berninger, Benedikt and Sandner, Beatrice and Bogdahn, Ulrich and Goetz, Magdalena and Bluemcke, Ingmar and Weidner, Norbert and Aigner, Ludwig (2009) Mesenchymal Stem Cells Promote Oligodendroglial Differentiation in Hippocampal Slice Cultures. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 24 (3-4). pp. 317-324. ISSN 1015-8987, 1421-9778
Full text not available from this repository. (Request a copy)Abstract
We have previously shown that soluble factors derived from mesenchymal stem cells (MSCs) induce oligodendrogenic fate and differentiation in adult rat neural progenitors (NPCs) in vitro. Here, we investigated if this pro-oligodendrogenic effect is maintained after cells have been transplanted onto rat hippocampal slice cultures, a CNS-organotypic environment. We first tested whether NPCs, that were pre-differentiated in vitro by MSC-derived conditioned medium, would generate oligodendrocytes after transplantation. This approach resulted in the loss of grafted NPCs, suggesting that oligodendroglial pre-differentiated cells could not integrate in the tissue and therefore did not survive grafting. However, when NPCs together with MSCs were transplanted in situ into hippocampal slice cultures, the grafted NPCs survived and the majority of them differentiated into oligodendrocytes. In contrast to the prevalent oligodendroglial differentiation in case of the NPC/MSC co-transplantation, naive NPCs transplanted in the absence of MSCs differentiated predominantly into astrocytes. In summary, the pro-oligodendrogenic activity of MSCs was maintained only after co-transplantation into hippocampal slice cultures. Therefore, in the otherwise astrogenic milieu, MSCs established an oligodendrogenic niche for transplanted NPCs, and thus, co-transplantation of MSCs with NPCs might provide an attractive approach to re-myelinate the various regions of the diseased CNS. Copyright (C) 2009 S. Karger AG, Basel
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CENTRAL-NERVOUS-SYSTEM; ORGANOTYPIC CULTURES; REMYELINATION; TRANSPLANTATION; PROLIFERATION; REPAIR; MODEL; IMMUNOSUPPRESSION; DEMYELINATION; DISEASE; Mesenchymal stem cells; Neural progenitors; Oligodendrocyte differentiation; Hippocampal slice culture; Multiple Sclerosis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Oct 2020 08:17 |
| Last Modified: | 09 Oct 2020 08:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29659 |
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