Wagner, Katrin and Couillard-Despres, Sebastien and Lehner, Bernadette and Brockhoff, Gero and Rivera, Francisco J. and Blume, Annegret and Neumann, Inga and Aigner, Ludwig (2009) Prolactin Induces MAPK Signaling in Neural Progenitors without Alleviating Glucocorticoid-Induced Inhibition of in vitro Neurogenesis. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 24 (5-6). pp. 397-406. ISSN 1015-8987, 1421-9778
Full text not available from this repository. (Request a copy)Abstract
We recently demonstrated that prolactin (PRL) prevents chronic stress-induced inhibition of adult hippocampal neurogenesis. It remained unsettled, however, whether PRL is acting directly on neural stem and progenitors cells (NPCs) or if neurogenesis is affected by an indirect mechanism, for example through the extensively described effects of PRL on the HPA axis. To address this point, we used neurosphere cultures derived from the adult rat hippocampus as an in vitro model for NPCs. Dexamethasone (DEX) was applied to stress the NPCs, and proliferation, survival and differentiation of cells were examined. DEX markedly inhibited proliferation of NPCs and cells entered the G 0 phase of cell cycle. Moreover, DEX reduced NPC survival and repressed astroglial differentiation, which is normally induced by serum or bone morphogenetic protein application. Even though we could demonstrate that NPCs express the PRL receptor and ERK1/2 signaling is induced by PRL, we did not observe any effect of PRL on NPCs proliferation, differentiation or survival, neither in the presence nor during absence of DEX. In summary, our results indicate that PRL action on NPCs and neurogenesis in vivo occurs via an indirect mechanism. Copyright (C) 2009 S. Karger AG, Basel
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ADULT HIPPOCAMPAL NEUROGENESIS; RAT DENTATE GYRUS; STEM-CELLS; CORTICOSTEROID RECEPTORS; PSYCHOSOCIAL STRESS; NEURONAL FATE; GROWTH-FACTOR; DEXAMETHASONE; PROLIFERATION; EXPRESSION; Neural stem cells; Neural progenitors; Neuropeptides; Stress; Cell proliferation; Astroglia |
| Subjects: | 500 Science > 590 Zoological sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) Medicine > Lehrstuhl für Neurologie Biology, Preclinical Medicine > Institut für Zoologie > Tierphysiologie/Neurobiologie (Prof. Dr. Inga Neumann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Oct 2020 05:25 |
| Last Modified: | 12 Oct 2020 05:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29661 |
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