Schreml, Stephan and Babilas, Philipp and Fruth, Sabine and Orso, Evelyn and Schmitz, Gerd and Mueller, Michael B. and Nerlich, Michael and Prantl, Lukas (2009) Harvesting human adipose tissue-derived adult stem cells: resection versus liposuction. CYTOTHERAPY, 11 (7). pp. 947-957. ISSN 1465-3249, 1477-2566
Full text not available from this repository. (Request a copy)Abstract
Background Adipose tissue is an abundant source of mesenchymal stem cells (MSC), which can be used for tissue-engineering purposes. The aim of our study was to determine the more suitable procedure, surgical resection or liposuction, for harvesting human adipose tissue-derived stem cells (hASC) with regard to viability, cell count and differentiation potential. Methods After harvesting hASC, trypan blue staining and cell counting were carried out. Subsequently, hASC were cultured, analyzed by fluorescence-activated cell sorting (FACS) and differentiated under adipogenic, osteogenic and chondrogenic conditions. Histologic and functional analyzes were performed at the end of the differentiation period. Results No significant difference was found with regard to the cell counts of hASC from liposuction and surgically resected material (P = 0.086). The percentage of viable cells was significantly higher for liposuction aspirates than for resection material (P = 0.002). No significant difference was found in the adipogenic differentiation potential (P = 0.179). A significantly lower number of cultures obtained from liposuction material than from resection material could be differentiated into osteocytes (P = 0.049) and chondrocytes (P = 0.012). Discussion Even though some lineages from lipoaspirated hASC can not be differentiated as frequently as those from surgically resected material, liposuction may be superior for some tissue-engineering purposes, particularly because of the less invasive harvesting procedure, the higher percentage of viable cells and the fact that there is no significant difference between lipoaspirated and resected hASC with regard to adipogenic differentiation potential.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROCESSED LIPOASPIRATE CELLS; PROGENITOR CELLS; STROMAL CELLS; FAT PAD; DIFFERENTIATION; YIELD; CARTILAGE; LIPOTRANSFER; REGENERATION; THERAPIES; adipose tissue; differentiation; liposuction; mesenchymal stem cells; resection; tissue reconstruction |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Plastische-, Hand- und Wiederherstellungschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Oct 2020 08:28 |
| Last Modified: | 12 Oct 2020 08:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29704 |
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