Lipscomb, Gina L. and Keese, Annette M. and Cowart, Darin M. and Schut, Gerrit J. and Thomm, Michael and Adams, Michael W. W. and Scott, Robert A. (2009) SurR: a transcriptional activator and repressor controlling hydrogen and elemental sulphur metabolism in Pyrococcus furiosus. MOLECULAR MICROBIOLOGY, 71 (2). pp. 332-349. ISSN 0950-382X, 1365-2958
Full text not available from this repository. (Request a copy)Abstract
This work describes the identification and characterization of SurR, Pyrococcus furiosus sulphur (S-0) response regulator. SurR was captured from cell extract using promoter DNA of a hydrogenase operon that is downregulated in the primary response of P. furiosus to S-0, as revealed by DNA microarray experiments. SurR was validated as a sequence-specific DNA binding protein, and characterization of the SurR DNA binding motif GTTn(3)AAC led to the identification of several target genes that contain an extended motif in their promoters. A number of these were validated to contain upstream SurR binding sites. These SurR targets strongly correspond with open reading frames and operons both up- and downregulated in the primary response to S-0. In vitro transcription revealed that SurR is an activator for its own gene as well as for two hydrogenase operons whose expression is downregulated during the primary S-0 response; it is also a repressor for two genes upregulated during the primary S-0 response, one of which encodes the primary S-0-reducing enzyme NAD(P)H sulphur reductase. Herein we give evidence for the role of SurR in both mediating the primary response to S-0 and controlling hydrogen production in P. furiosus.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN DISULFIDE OXIDOREDUCTASE; CELL-FREE TRANSCRIPTION; ARCHAEAL TRANSCRIPTION; RNA-POLYMERASE; IDENTIFICATION; REGULATOR; RECRUITMENT; SPECIFICITY; PROMOTER; COMPLEX; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Oct 2020 14:50 |
| Last Modified: | 13 Oct 2020 14:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29810 |
Actions (login required)
 |
View Item |
