Grosse, Jirka and Grimm, Daniela and Westphal, Kriss and Ulbrich, Claudia and Moosbauer, Jutta and Pohl, Fabian and Koelbl, Oliver and Infanger, Manfred and Eilles, Christoph and Schoenberger, Johann (2009) Radiolabeled annexin V for imaging apoptosis in radiated human follicular thyroid carcinomas - is an individualized protocol necessary? NUCLEAR MEDICINE AND BIOLOGY, 36 (1). pp. 89-98. ISSN 0969-8051,
Full text not available from this repository. (Request a copy)Abstract
Introduction: Induction of apoptosis is a widely used strategy for cancer therapy, but evaluating the degree and success of this therapy still poses a problem. Radiolabeled annexin V has been proposed to be a promising candidate for detecting apoptotic cells in tumors following chemotherapy in vivo. In order to see whether radiolabeled annexin V Could be a suitable substance for the noninvasive in vivo detection of apoptosis in thyroid tissue and to establish an optimized study protocol, we investigated two poorly differentiated thyroid carcinoma cell lines: ML-1 and FTC-133. Methods: Apoptosis was evaluated before as well as 2 and 4 days after in vitro irradiation with 30 Gy X-rays. In this study, binding of FITC- and of (125)I-labeled annexin V was measured in comparison to other apoptosis arkers such as Bax, caspase-3 and Fas, which were determined by flow cytometry and Western blot analysis with densitometric evaluation. Results: ML-1 and FTC-133 cells showed a significant increase in annexin V binding 48 h after irradiation. Ninety-six hours after irradiation, the annexin V absorption capability of ML-1 cells was still maximal, while the living fraction of FTC-133 increased significantly. The amount of caspase-3 and Bax was clearly increased 48 h after irradiation and had normalized after 96 h in both cell lines. Fas protein concentrations remained unchanged in ML-1 cells but were significantly enhanced in FTC-133 cells. Conclusion: The binding of FITC- and (125)I-labeled annexin V showed a significant accordance. A reliable evaluation of apoptosis induced by radiotherapy in thyroid tumors was possible 48 It after irradiation, when binding of radiolabeled annexin V is most significantly enhanced. Using two poorly differentiated cell lines of thyroid carcinoma, one may expect to find a nearly similar response to external irradiation. In contrast, the cell lines showed a completely contrary response. However, an individualized study protocol for each type of tumor and probably within each type is necessary. (C) 2009 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IN-VIVO DETECTION; SIMULATED MICROGRAVITY; PROLIFERATIVE ACTIVITY; TUMOR APOPTOSIS; CELL-DEATH; TC-99M-ANNEXIN-V; ASYMMETRY; PEPTIDES; GROWTH; MODEL; Annexin V; Apoptosis; Molecular imaging; Thyroid cancer; Irradiation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Strahlentherapie Medicine > Abteilung für Nuklearmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Oct 2020 08:24 |
| Last Modified: | 13 Oct 2020 08:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29821 |
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