Kele, Peter and Li, Xiaohua and Link, Martin and Nagy, Krisztina and Herner, Andras and Lorincz, Krisztian and Beni, Szabolcs and Wolfbeis, Otto S. (2009) Clickable fluorophores for biological labeling-with or without copper. ORGANIC & BIOMOLECULAR CHEMISTRY, 7 (17). pp. 3486-3490. ISSN 1477-0520,
Full text not available from this repository. (Request a copy)Abstract
The synthesis of a set of new clickable fluorophores that virtually cover the whole visible spectrum reaching the near infra-red regime is presented herein. Besides dyes that are capable of participating in classical copper catalyzed 1,3-dipolar cycloaddition reactions with the counterparting function we have also prepared dyes containing a cyclooctyne moiety, an alkyne derivative that enables copper free clicking to azides. The suitability of these dyes for fluorescent labeling of biomolecules is presented by examples on model frameworks representing major biopolymer building blocks. The versatility of these dyes is presented in cell labeling experiments as well as by labeling the azide modified surface glycans of CHO-cells either by copper catalyzed or copper-free click reaction. These dyes are expected to have a large variety of applications in (bio)orthogonal labeling schemes both in vivo and in vitro.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 1,3-DIPOLAR CYCLOADDITIONS; TERMINAL ALKYNES; LIVING SYSTEMS; CHEMISTRY; LIGATION; AZIDES; PROTEINS; GLYCANS; CELLS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Oct 2020 14:51 |
| Last Modified: | 13 Oct 2020 14:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29826 |
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