Ramm, Paul and Couillard-Despres, Sebastien and Ploetz, Sonja and Rivera, Francisco J. and Krampert, Monika and Lehner, Bernadette and Kremer, Werner and Bogdahn, Ulrich and Kalbitzer, Hans R. and Aigner, Ludwig (2009) A Nuclear Magnetic Resonance Biomarker for Neural Progenitor Cells: Is It All Neurogenesis? STEM CELLS, 27 (2). pp. 420-423. ISSN 1066-5099, 1549-4918
Full text not available from this repository. (Request a copy)Abstract
In vivo visualization of endogenous neural progenitor cells (NPCs) is crucial to advance stem cell research and will be essential to ensure the safety and efficacy of neurogenesis-based therapies. Magnetic resonance spectroscopic imaging (i.e., spatially resolved spectroscopy in vivo) is a highly promising technique by which to investigate endogenous neurogenesis noninvasively. A distinct feature in nuclear magnetic resonance spectra (i.e., a lipid signal at 1.28 ppm) was recently attributed specifically to NPCs in vitro and to neurogenic regions in vivo. Here, we demonstrate that although this 1.28-ppm biomarker is present in NPC cultures, it is not specific for the latter. The 1.28-ppm marker was also evident in mesenchymal stem cells and in non-stem cell lines. Moreover, it was absent in freshly isolated NPCs but appeared under conditions favoring growth arrest or apoptosis; it is initiated by induction of apoptosis and correlates with the appearance of mobile lipid droplets. Thus, although the 1.28-ppm signal cannot be considered as a specific biomarker for NPCs, it might still serve as a sensor for processes that are tightly associated with neurogenesis and NPCs in vivo, such as apoptosis or stem cell quiescence. However, this requires further experimental evidence. The present work clearly urges the identification of additional biomarkers for NPCs and for neurogenesis. STEM CELLS 2009; 27: 420-423
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MOBILE LIPIDS; DEATH; NMR; APOPTOSIS; DOUBLECORTIN; NEURONS; BODIES; GROWTH; Imaging; Neural stem cells; Mesenchymal stem cells; Lipid droplets |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Oct 2020 05:35 |
| Last Modified: | 14 Oct 2020 05:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29889 |
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