Grossmann, Guido and Malinsky, Jan and Stahlschmidt, Wiebke and Loibl, Martin and Weig-Meckl, Ina and Frommer, Wolf B. and Opekarova, Miroslava and Tanner, Widmar (2008) Plasma membrane microdomains regulate turnover of transport proteins in yeast. JOURNAL OF CELL BIOLOGY, 183 (6). pp. 1075-1088. ISSN 0021-9525, 1540-8140
Full text not available from this repository. (Request a copy)Abstract
In this study, we investigate whether the stable segregation of proteins and lipids within the yeast plasma membrane serves a particular biological function. We show that 21 proteins cluster within or associate with the ergosterol-rich membrane compartment of Can1 (MCC). However, proteins of the endocytic machinery are excluded from MCC. In a screen, we identified 28 genes affecting MCC appearance and found that genes involved in lipid biosynthesis and vesicle transport are significantly over-represented. Deletion of Pil1, a component of eisosomes, or of Nce102, an integral membrane protein of MCC, results in the dissipation of all MCC markers. These deletion mutants also show accelerated endocytosis of MCC-resident permeases Can1 and Fur4. Our data suggest that release from MCC makes these proteins accessible to the endocytic machinery. Addition of arginine to wild-type cells leads to a similar redistribution and increased turnover of Can1. Thus, MCC represents a protective area within the plasma membrane to control turnover of transport proteins.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; CELL-SURFACE; LIPID RAFTS; URACIL PERMEASE; BUDDING YEAST; PKH-KINASES; ENDOCYTOSIS; DOMAINS; SPHINGOLIPIDS; PATHWAY; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Oct 2020 08:59 |
| Last Modified: | 14 Oct 2020 08:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29928 |
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