Tyagi, A. and Penzkofer, Alfons and Griese, J. and Schlichting, I. and Kirienko, Natalia V. and Gomelsky, Mark (2008) Photodynamics of blue-light-regulated phosphodiesterase BlrP1 protein from Klebsiella pneumoniae and its photoreceptor BLUF domain. CHEMICAL PHYSICS, 354 (1-3). pp. 130-141. ISSN 0301-0104, 1873-4421
Full text not available from this repository. (Request a copy)Abstract
The BlrP1 protein from the enteric bacterium Klebsiella pneumoniae consists of a BLUF and an EAL domain and may activate c-di-GMP phosphodiesterase by blue-light. The full-length protein, BlrP1, and its BLUF domain, BlrP1_BLUF, are characterized by optical absorption and emission spectroscopy. The cofactor FAD in its oxidized redox state (FAD(ox)) is brought from the dark-adapted receptor state to the 10-nm red-shifted Putative signalling state by violet light exposure. The recovery to the receptor state Occurs with a time constant of about I min. The quantum yield of signalling state formation is about 0.17 for BlrP1_BLUF and about 0.08 for BlrP1. The fluorescence efficiency of the FAD(ox) cofactor is small due to photo-induced reductive electron transfer. Prolonged light exposure converts FAD(ox) in the signalling state to the fully reduced hydroquinone form FAD(red)H(-) and causes low-efficient chromophore release with subsequent photo-degradation. The photo-cycle and photo-reduction dynamics in the receptor state and in the signalling state are discussed. (C) 2008 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SYNECHOCYSTIS SP PCC6803; THERMOSYNECHOCOCCUS-ELONGATUS BP-1; INDUCED STRUCTURAL-CHANGES; FLUORESCENCE SPECTROSCOPIC CHARACTERIZATION; QUANTUM-CHEMICAL CALCULATIONS; FLAVIN-BINDING PHOTORECEPTOR; MEDIATED SIGNAL-TRANSDUCTION; RHODOBACTER-SPHAEROIDES; AQUEOUS-SOLUTION; HYDROGEN-BOND; BLUF domain; Blue-light-regulated phosphodiesterase (BlrP1); Klebsiella pneumoniae; Blue-light receptor; FAD; Absorption spectroscopy; Fluorescence spectroscopy; Photo-cycle; Photo-induced electron transfer; Photo-reduction |
| Subjects: | 500 Science > 530 Physics |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Oct 2020 09:29 |
| Last Modified: | 14 Oct 2020 09:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29932 |
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