Speth, Cornelia and Bredl, Simon and Hagleitner, Magdalena and Wild, Jens and Dierich, Manfred and Wolf, Hans and Schroeder, Josef and Wagner, Ralf and Deml, Ludwig (2008) Human immunodeficiency virus type-1 (HIV-1) Pr55(gag) virus-like particles are potent activators of human monocytes. VIROLOGY, 382 (1). pp. 46-58. ISSN 0042-6822,
Full text not available from this repository. (Request a copy)Abstract
Human immunodeficiency virus type-1 (HIV-1) Pr55(Gag) virus-like particles (VLP) represent an interesting HIV vaccine component since they stimulate strong humoral and cellular immune responses. We demonstrated that VLP expressed by recombinant baculoviruses activate human PBMC to release pro-inflammatory (IL-6, TNF-alpha), anti-inflammatory (IL-10) and Th1-polarizing (IFN-gamma) cytokines as well as GM-CSF and MIP-1 alpha in a dose-and time-dependent manner. Herein, residual baculoviruses within the VLP preparations showed no or minor effects. Monocytes could be identified as a main target for Vu? to induce cytokine production. Furthermore, VLP-induced monocyte activation was shown by upregulation of molecules involved in antigen presentation (MHC11, CD80, CD86) and cell adhesion (CD54). Exposure of VLP to serum inactivates its capacity to stimulate cytokine production. In summary, these investigations establish VLP as strong activators of PBMC and monocytes therein, potently enhancing their functionality and potency to promote an efficient immune response. This capacity makes VLP an interesting component of combination vaccines. (C) 2008 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CYTOTOXIC T-CELLS; NEUTRALIZING ANTIBODIES; EXTRACELLULAR RELEASE; DENDRITIC CELLS; RHESUS MACAQUES; BACTERIAL-DNA; INSECT CELLS; GENE-PRODUCT; BACULOVIRUS; EXPRESSION; Virus-like particles; Cytokines; Innate immunity; Monocytes; PBMC |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Oct 2020 09:38 |
| Last Modified: | 14 Oct 2020 09:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29939 |
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