Schrenkhammer, Petra and Wolfbeis, Otto S. (2008) Fully reversible optical biosensors for uric acid using oxygen transduction. BIOSENSORS & BIOELECTRONICS, 24 (4). pp. 994-999. ISSN 0956-5663, 1873-4235
Full text not available from this repository. (Request a copy)Abstract
An optical biosensor is presented for continuous determination of uric acid. The scheme is based on the measurement of the consumption of oxygen during the oxidation of uric acid that is catalyzed by the enzyme uricase. The enzyme is immobilized in a polyurethane hydrogel next to a metal-organic probe whose fluorescence is quenched by oxygen. The consumption of oxygen was followed by measurement of changes of luminescence intensity of two kind of probes and can be related to the concentration of uric acid. Analytical ranges (0-2 mM), the response times (80-100 s), reproducibility, and long-term stability were investigated. The biosensors; are stable for at least 1 month and are not interfered by common interferents. One kind of biosensor was applied to the determination of uric acid in human blood serum. The results agree with those of a commercial colorimetric detection kit. (C) 2008 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ASSEMBLED GOLD ELECTRODE; GLASSY-CARBON ELECTRODE; ELECTROCHEMICAL-BEHAVIOR; ASCORBIC-ACID; COMPLEXES; SENSORS; SERUM; Uric acid; Fluorescence; Oxygen sensor; Oxygen probe; Enzymatic assay |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Oct 2020 09:45 |
| Last Modified: | 14 Oct 2020 09:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29945 |
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