Alexander, Stephanie and Koehl, Gudrun E. and Hirschberg, Markus and Geissler, Edward K. and Friedl, Peter (2008) Dynamic imaging of cancer growth and invasion: a modified skin-fold chamber model. HISTOCHEMISTRY AND CELL BIOLOGY, 130 (6). pp. 1147-1154. ISSN 0948-6143, 1432-119X
Full text not available from this repository. (Request a copy)Abstract
The metastatic invasion of cancer cells from the primary lesion into the adjacent stroma is a key step in cancer progression, and is associated with poor outcome. The principles of cancer invasion have been experimentally addressed in various in vitro models; however, key steps and mechanisms in vivo remain unclear. Here, we establish a modified skin-fold chamber model for orthotopic implantation, growth and invasion of human HT-1080 fibrosarcoma cells, dynamically reconstructed by epifluorescence and multiphoton microscopy. This strategy allows repeated imaging of tumor growth, tumor-induced angiogenesis and invasion, as either individual cells, or collective strands and cell masses that move along collagen-rich extracellular matrix and coopt host tissue including striated muscle strands and lymph vessels. This modified window model will be suited to address mechanisms of cancer invasion and metastasis, and related experimental therapy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IN-VIVO; PERICELLULAR PROTEOLYSIS; MESENCHYMAL TRANSITION; TUMOR ANGIOGENESIS; GENE-EXPRESSION; CELL-MIGRATION; METASTASIS; MICROSCOPY; MOVEMENT; EXPLANTS; Collective invasion; Dorsal skin-fold chamber; Fibrosarcoma; Multiphoton microscopy; Tumor microenvironment |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Oct 2020 10:13 |
| Last Modified: | 14 Oct 2020 10:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29960 |
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