Reidt, Ulrich and Chauhan, Lina and Mueller, Gerhard and Molz, Ramona and Lindner, Petra and Wolf, Hans and Friedberger, Alois (2008) REPRODUCIBLE FILTRATION OF BACTERIA WITH MICROMECHANICAL FILTERS. JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY, 16 (4). pp. 337-350. ISSN 1060-3999, 1745-4581
Full text not available from this repository. (Request a copy)Abstract
For the detection of bacteria in potable water, several kinds of biosensors could be used. However, any of them faces its physical limits when very low concentrations of bacteria should be detected. In this article, we used micromechanical filters with perforated membranes for capturing and enriching bacteria and to facilitate the detection of Escherichia coli. The results in this article show the easy and effective removal of bacteria from the surface of the microsieve membranes. By plating off the filters on LB agar plates, we could quantitatively analyze very low concentrations of bacteria and show that micromechanical filters are potentially suitable for subsequent transfer of bacteria to a biochip for further analysis. In addition, direct immunodetection of nonpathogenic E. coli O157:H7 on micromechanical filter surfaces by fluorescent-labelled polyclonal antibodies is demonstrated. In summary, micromechanical filters present a promising tool for future detection of bacteria in potable water.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ESCHERICHIA-COLI; DRINKING-WATER; PARTICLE FILTERS; MEMBRANE; ENUMERATION; SEPARATION; COLIFORMS; FOOD; OPTIMIZATION; CELLS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Oct 2020 10:49 |
| Last Modified: | 16 Oct 2020 10:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29987 |
Actions (login required)
 |
View Item |
