Escher, Felicitas and Modrow, Susanne and Sabi, Titus and Kuehl, Uwe and Lassner, Dirk and Schultheiss, Heinz-Peter and Noutsias, Michel (2008) Parvovirus B19 profiles in patients presenting with acute myocarditis and chronic dilated cardiomyopathy. MEDICAL SCIENCE MONITOR, 14 (12). CR589-CR597. ISSN 1643-3750,
Full text not available from this repository. (Request a copy)Abstract
Background: Parvovirus B19 (B19V) is the most prevalent cardiotropic Vir-LIS in endomyocardial biopsies (EMBs) from patients presenting with acute myocarditis (AMC) and chronic dilated cardiomyopathy (DCM). We elucidated the role of B19V specific IgG and IgM antibodies against native VP2-capsids and denatured VP1-, VP2- and NS1-proteins, which discriminate disease acuity in other B19V related diseases, in patients presenting with clinically suspected AMC and DCM for the determination of the viral infection stage. Material/Methods: n=62 prospectively enrolled,WC (n=33) and DCM (n=29) patients were investigated. B19V genomes were amplified in EMBs by nested PCR (nPCR). B19V-specific IgG and IgM were investigated by recomLine (R) blots in the sera. Results: B19V genomes were detectable by nPCR with comparable frequencies in AMC (63.6%) and DCM patients (51.7%), respectively. IgM antibodies were detectable in 18.1% of the AMC, but not in DCM patients. In patients with myocardial B19V infection, antibody profiles indicating recent infections were more frequent in WC (47.6%) compared with DCM patients (6.7%), while persistent/reactivating profiles were significantly more prevalent in DCM (20.0%) compared with AMC (0%) patients (p<0.05). Conclusions: IgM B19V antibodies can be detected primarily in AMC, but not in DCM patients. The discrimination of the B19V specific IgG antibodies using recomLine (R) blots may be suitable to distinguish acute versus chronic, and persistent/reactivating infection status in patients with myocardial B19V infection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOMYOCARDIAL BIOPSY SPECIMENS; NONSTRUCTURAL PROTEIN; MAGNETIC-RESONANCE; RHEUMATIC-DISEASE; VIRAL PERSISTENCE; INFECTED PATIENTS; CAPSID PROTEINS; ANTIBODIES; IGG; INDIVIDUALS; myocarditis; dilated cardiomyopathy; inflammatory cardiomyopathy; Parvovirus B19; humoral immune response; diagnosis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Oct 2020 10:57 |
| Last Modified: | 16 Oct 2020 10:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29999 |
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