Haas, Philipp and Straub, Rainer H. and Bedoui, Sammy and Nave, Heike (2008) Peripheral but not central leptin treatment increases numbers of circulating NK cells, granulocytes and specific monocyte subpopulations in non-endotoxaemic lean and obese LEW-rats. REGULATORY PEPTIDES, 151 (1-3). pp. 26-34. ISSN 0167-0115, 1873-1686
Full text not available from this repository. (Request a copy)Abstract
Leptin, a hormone mainly generated by adipocytes, acts centrally in the hypothalamus to regulate body weight and energy expenditure. However, there is strong evidence that leptin is also involved in cell-mediated immunity and cytokine crosstalk. In the present study the effects of diet-induced obesity and central and peripheral leptin treatment on leukocyte subsets and cytokine production was investigated. Leptin was injected either intravenously (i.v.) or intracerebroventricularly (i.c.v.) in male endotoxaemic or vehicle-treated healthy LEW-rats. Numbers of blood leukocyte subsets were analysed by FACS and cytokines (TNF-alpha and IL-6) by ELISA. Results showed that peripheral rather than central leptin treatment was able to significantly increase numbers of granulocytes. NK cells and monocytes. Three-colour staining revealed that the increase of ED9(+) monocytes was most likely due to the mobilization of two distinct monocyte subsets. predominantly ED9(+)CD4(-)NKR-P1A(+) and ED9(+)CD4(+)NKR-P1A(+). ELISA analysis revealed significantly elevated TNF-a levels in obese animals compared to their lean littermates, while IL-6 failed to show notable changes. In conclusion. the data of the present study revealed that leptin application induces a nutrition- and application-site dependent increase of circulating NK cells. granulocytes and specific monocyte subsets. (C) 2008 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SYMPATHETIC-NERVOUS-SYSTEM; NEUROPEPTIDE-Y; ADIPOSE-TISSUE; IN-VIVO; ACTIVATION; BLOOD; RESISTANCE; MICE; PROLIFERATION; INFLAMMATION; Leptin; NK cells; Monocytes; Activated monocytes; TNF-alpha; Endotoxaemia; Obesity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Oct 2020 05:43 |
| Last Modified: | 19 Oct 2020 05:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30033 |
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