May, Florian and Matiasek, Kaspar and Vroemen, Maurice and Caspers, Christiane and Mrva, Thomas and Arndt, Christian and Schlenker, Boris and Gais, Peter and Brill, Thomas and Buchner, Alexander and Blesch, Armin and Hartung, Rudolf and Stief, Christian and Gansbacherf, Bernd and Weidner, Norbert (2008) GDNF-Transduced Schwann Cell Grafts Enhance Regeneration of Erectile Nerves. EUROPEAN UROLOGY, 54 (5). pp. 1179-1187. ISSN 0302-2838,
Full text not available from this repository. (Request a copy)Abstract
Background: Schwann cell-seeded guidance tubes have been shown to promote cavernous nerve regeneration, and the local delivery of neurotrophic factors may additionally enhance nerve regenerative capacity. The present study evaluates whether the transplantation of GDNF-overexpressing Schwann cells may enhance regeneration of bilaterally transected erectile nerves in rats. Methods: Silicon tubes seeded with either GDNF-overexpressing or GFP-expressing Schwann cells were implanted into the gaps between transected cavernous nerve endings. Six (10 study nerves) or 12 wk (20 study nerves) postoperatively, erectile function was evaluated by relaparotomy, electrical nerve stimulation, and intracavernous pressure recording, followed by ultrastructural evaluation of reconstructed nerves employing blight-field and electron microscopy. Additional animals were either sham-operated (positive control; 20 study nerves) or received bilateral nerve transection without nerve reconstruction (negative control; 20 study nerves). Results: The combination of GDNT delivery and Schwann cell application promoted an intact erectile response in 90% (9 of 10) of grafted nerves after 6 wk and in 95% (19 of 20) after 12 wk, versus 50% (5 of 10) and 80% (16 of 20) of GFP-expressing Schwann cell grafts (p = 0.02). The functional recovery was paralleled by enhanced axonal regeneration in GDNF-overexpressing Schwann cell grafts, as indicated by larger cross-sectional areas and a significantly higher percentage of neural tissue compared with GFP-transduced controls. Conclusions: These findings demonstrate that the time required to elicit functional recovery of erectile nerves can be reduced by local delivery of GDNF. In terms of clinical application, this enhanced nerve repair might be critical for timely reinnervation of the corpus cavernosum as a prerequisite for functional recovery in men. (C) 2008 European Association of Urology. Published by Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NEUROTROPHIC FACTOR; SPINAL-CORD; AXONAL GROWTH; PERIPHERAL-NERVE; MOTOR-NEURONS; ADULT-RAT; IN-VIVO; EXPRESSION; SURVIVAL; DELIVERY; Erection; Nerve grafts; Schwann cells; GDNF; Neurotrophic factor; Gene therapy |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Oct 2020 09:42 |
| Last Modified: | 19 Oct 2020 09:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30095 |
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