Sinha, Himanshu and David, Lior and Pascon, Renata C. and Clauder-Muenster, Sandra and Krishnakumar, Sujatha and Nguyen, Michelle and Shi, Getao and Dean, Jed and Davis, Ronald W. and Oefner, Peter J. and McCusker, John H. and Steinmetz, Lars A. (2008) Sequential Elimination of Major-Effect Contributors Identifies Additional Quantitative Trait Loci Conditioning High-Temperature Growth in Yeast. GENETICS, 180 (3). pp. 1661-1670. ISSN 0016-6731, 1943-2631
Full text not available from this repository. (Request a copy)Abstract
Several quantitative trait loci (QTL) mapping strategies can successfully identify major-effect loci, but often have poor success detecting loci with minor effects, potentially due to the confounding effects of major loci, epistasis, and limited sample sizes. To overcome such difficulties, we used a targeted backcross mapping strategy that genetically eliminated the effect of a previously identified major QTL underlying high-temperature growth (Htg) in yeast. This strategy facilitated the mapping of three novel QTL contributing to Htg of a clinically derived yeast strain. One QTL, which is linked to the previously identified major-effect QTL, was dissected, and NCS2 was identified as the causative gene. The interaction of the NCS2QTL with the first major-effect QTL was background dependent, revealing a complex QTL architecture spanning these two linked loci. Such complex architecture suggests that more genes can be predicted are likely to contribute to quantitative traits. The targeted backcrossing approach overcomes the difficulties posed by sample size, genetic linkage, and epistatic effects and facilitates identification of additional alleles with smaller contributions to complex traits.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; GENETIC DISSECTION; COMPLEX TRAITS; BUDDING YEAST; RESOLUTION; GENOME; EPISTASIS; TRANSCRIPTION; EXPRESSION; DROSOPHILA; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Oct 2020 10:00 |
| Last Modified: | 19 Oct 2020 10:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30097 |
Actions (login required)
 |
View Item |
