Barabas, Sascha and Gary, Regina and Bauer, Tanja and Lindner, Juha and Lindner, Petra and Weinberger, Birgit and Jilg, Wolfgang and Wolf, Hans and Deml, Ludwig (2008) Urea-Mediated Cross-Presentation of Soluble Epstein-Barr Virus BZLF1 Protein. PLOS PATHOGENS, 4 (11): e1000198. ISSN 1553-7366,
Full text not available from this repository. (Request a copy)Abstract
Soluble extracellular proteins usually do not enter the endogenous human leukocyte antigen (HLA) I-dependent presentation pathway of antigen-presenting cells, strictly impeding their applicability for the re-stimulation of protein-specific CD8(+) cytotoxic T lymphocytes (CTL). Here we present for the Epstein-Barr virus (EBV) BZLF1 a novel strategy that facilitates protein translocation into antigen-presenting cells by its solubilisation in high molar urea and subsequent pulsing of cells in presence of low molar urea. Stimulation of PBMC from HLA-matched EBV-seropositive individuals with urea-treated BZLF1 but not untreated BZLF1 induces an efficient reactivation of BZLF1-specific CTL. Urea-treated BZLF1 (uBZLF1) enters antigen-presenting cells in a temperature-dependent manner by clathrin-mediated endocytosis and is processed by the proteasome into peptides that are bound to nascent HLA I molecules. Dendritic cells and monocytes but also B cells can cross-present uBZLF1 in vitro. The strategy described here has potential for use in the development of improved technologies for the monitoring of protein-specific CTL.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CLASS-I PRESENTATION; T-CELL RESPONSES; TAT-FUSION PROTEINS; DENDRITIC CELLS; EXOGENOUS ANTIGEN; ADAPTIVE IMMUNITY; NUCLEAR ANTIGEN; TRANSDUCTION; LYMPHOCYTES; MOLECULES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Oct 2020 08:26 |
| Last Modified: | 21 Oct 2020 08:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30141 |
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