Leidl, Katharina and Liebisch, Gerhard and Richter, Dorothea and Schmitz, Gerd (2008) Mass spectrometric analysis of lipid species of human circulating blood cells. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1781 (10). pp. 655-664. ISSN 1388-1981,
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Circulating blood cell lipid composition may become increasingly important to provide new insights into cellular lipid abnormalities in diseases. Here we compared lipid species in monocytes, lymphocytes, granulocytes, platelets and red blood cells (RBC) of healthy volunteers using electrospray ionization tandem mass spectrometry and detected striking differences among the examined blood cells. The different cell types were characterized by unique lipid class and lipid species pattern. The predominant lipid classes were phosphatidylcholine (PC) and free cholesterol (FC) with cell type specific PC/FC ratios as markers of membrane fluidity which was 1.9 in monocytes, 1.3 in lymphocytes, 1.1 in granulocytes, 0.8 in platelets and 0.3 in RBC, respectively. Beside a three-fold elevated ceramide level of 2.6 mol%, granulocytes revealed the highest percentage of phosphatidylethanolamine-based plasmalogens and a decreased fraction of highly polyunsaturated ( :3 double bonds) species compared to other cell types. Furthermore RBC showed a remarkable shift of glycerophospholipid chain length and platelets a nearly 4-fold increase of the cholesterol ester (CE) 18:2 (linoleic acid) fraction (55 mol% of total CE). In conclusion, the current study is a detailed comparison of lipid species in circulating blood cells of healthy human donors. This work could be a reference for studies in different patient cohorts directed towards discovery of novel lipid biomarkers in circulating blood cells. (c) 2008 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FATTY-ACID-COMPOSITION; HIGH-THROUGHPUT QUANTIFICATION; HUMAN-LIVER DISEASE; QUANTITATIVE-ANALYSIS; MONONUCLEAR-CELLS; HUMAN-PLATELETS; ESI-MS/MS; PHOSPHOLIPIDS; PLASMA; ERYTHROCYTES; Monocytes; Lymphocytes; Granulocytes; Platelets; Red blood cell; ESI-MS/MS; Phospholipid; Lipidomics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Oct 2020 07:12 |
| Last Modified: | 22 Oct 2020 07:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30191 |
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