Wurm, Sylvia and Neumeier, Markus and Weigert, Johanna and Wanninger, Josef and Geri, Melanie and Gindner, Antonia and Schaeffler, Andreas and Aslanidi, Charalampos and Schoelmerich, Juergen and Buechler, Christa (2008) Insulin induces monocytic CXCL8 secretion by the mitogenic signalling pathway. CYTOKINE, 44 (1). pp. 185-190. ISSN 1043-4666,
Full text not available from this repository. (Request a copy)Abstract
Oral glucose uptake alters the function of immune cells and an elevation of systemic CXCL8 was described. Monocytes secrete high amounts of CXCL8 and therefore it was analyzed whether glucose or insulin upregulate monocytic CXCL8 release. Incubation of monocytes with insulin for 2 h induced CXCL8 mRNA and secretion whereas glucose had no effect. Inhibition of the phosphatidylinositol 3-kinase by wortmannin or the mammalian target of rapamycin by rapamycin did not influence insulin-mediated CXCL8 induction. In contrast, blockage of the ERK-specific MAP kinase MEK with PD98059, that prevents phosphorylation of ERK1/ERK2, abrogated insulin-induced CXCL8 release in primary monocytes. To investigate the in vivo effect of oral glucose uptake, monocytes of healthy probands were isolated in the fasted state and 2 h after glucose ingestion and CXCL8 mRNA and protein were increased in the latter. CXCL8 was also higher when determined in the cell lysate of leukocytes 2 h after glucose uptake whereas plasma CXCL8 levels were significantly reduced. In summary, these data indicate that oral glucose uptake in insulin-sensitive adults is associated with elevated monocytic and reduced systemic CXCL8. (C) 2008 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACUTE HYPERGLYCEMIA; REGULATES MONOCYTE; HUMAN MACROPHAGES; INTERLEUKIN-8; EXPRESSION; INHIBITOR; GLUCOSE; ALPHA; NEUTROPHILS; ADHESION; Chemokine; Blood monocyte; Insulin; Oral glucose uptake |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Oct 2020 08:45 |
| Last Modified: | 22 Oct 2020 08:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30205 |
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