Sirianant, Lalida and Wanitchakool, Podchanart and Ousingsawat, Jiraporn and Benedetto, Roberta and Zormpa, Anna and Cabrita, Ines and Schreiber, Rainer and Kunzelmann, Karl (2016) Non-essential contribution of LRRC8A to volume regulation. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 468 (5). pp. 805-816. ISSN 0031-6768, 1432-2013
Full text not available from this repository. (Request a copy)Abstract
Volume regulation is an essential property of any living cell and needs to be tightly controlled. While different types of K+ channels have been found to participate in the regulation of cell volume, the newly identified volume-regulated anion channel (VRAC) LRRC8 has been claimed to be essential for volume regulation. In unbiased genome-wide small interfering RNA (siRNA) screens, two independent studies identified LRRC8A/Swell1 as an essential component of VRAC, thus being indispensable for cellular volume regulation. We reanalyzed the role of LRRC8A for VRAC and regulatory volume decrease (RVD) in several cell types and under various conditions. While the role of LRRC8A for VRAC and its contribution to RVD is confirmed, we find that it is not essential for swelling-activated anion currents or cellular volume regulation, or apoptotic cell shrinkage. The contribution of LRRC8A is variable and largely depending on the cell type.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ESSENTIAL COMPONENT; ANION CHANNEL; CELLS; DECREASE; CHLORIDE; ACTIVATION; EPITHELIUM; PROTEIN; CRYPTS; VRAC; LRRC8A; VRAC; Volume-activated anion channels; Volume regulation: regulatory volume decrease; RVD, apoptotic volume decrease; AVD |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Apr 2019 06:05 |
| Last Modified: | 05 Apr 2019 06:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3031 |
Actions (login required)
 |
View Item |
