Sasahira, Tomonori and Kirita, Tadaaki and Oue, Naohide and Bhawal, Ujjal Kumar and Yamamoto, Kazuhiko and Fujii, Kiyomu and Ohmori, Hitoshi and Luo, Yi and Yasui, Wataru and Bosserhoff, Anja Katrin and Kuniyasu, Hiroki (2008) High mobility group box-1-inducible melanoma inhibitory activity is associated with nodal metastasis and lymphangiogenesis in oral squamous cell carcinoma. CANCER SCIENCE, 99 (9). pp. 1806-1812. ISSN 1349-7006,
Full text not available from this repository. (Request a copy)Abstract
Melanoma inhibitory activity (MIA) is an 11-kDa secretory protein isolated from malignant melanoma cells that is correlated with invasion and metastasis in various human malignancies. We examined MIA expression in 62 oral squamous cell carcinomas (OSCC) by immunohistochemistry. MIA expression was significantly associated with nodal metastasis (P = 0.00018). MIA expression was also associated with expression of high mobility group box-1 (HMGB1) (P < 0.0001) and lymph vessel density (P < 0.0001). Expression levels of MIA, HMGB1, nuclear factor kB (NFkB) p65 and HMGB1-NFkB p65 binding were significantly higher in a metastatic human OSCC cell line (HSC3) than those in a non-metastatic OSCC cell line (HSC4). Treatment with receptor for advanced glycation end products (RAGE) antisense or small interfering RNA and human recombinant HMGB1 (hrHMGB1) did not affect MIA expression, whereas HMGB1 antisense or siRNA treatment decreased MIA expression in HSC3 cells. Then HMGB1 enhanced MIA expression as an NFkB cofactor but not as a RAGE ligand. MIA neutralization by MIA antibodies increased extracellular signal-related kinase 1/2 phosphorylation, but decreased p38 phosphorylation and the expression of vascular epithelial growth factor (VEGF)-C and -D. Treatment with p38 inihibitor decreased VEGF-C and -D expression in HSC3 cells. These results suggest that MIA expression is enhanced by the interaction of intracellular HMGB1 and NFkBp65 and MIA is closely involved in tumor progression and nodal metastasis by the increments of VEGF-C and VEGF-D in OSCC.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLYCATION END-PRODUCTS; MALIGNANT-MELANOMA; COLON-CANCER; ACTIVITY MIA; EXPRESSION; AMPHOTERIN; RECEPTOR; GROWTH; GENE; MACROPHAGES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Oct 2020 08:57 |
| Last Modified: | 26 Oct 2020 08:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30321 |
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