Sommer, Florian and Pollinger, Klaus and Brandl, Ferdinand and Weiser, Barbara and Tessmar, Joerg and Blunk, Torsten and Goepferich, Achim (2008) Hyalocyte proliferation and ECM accumulation modulated by bFGF and TGF-beta 1. GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 246 (9). pp. 1275-1284. ISSN 0721-832X,
Full text not available from this repository. (Request a copy)Abstract
Purpose In cases of severe retinal diseases, the vitreous body has to be removed and replaced by a suitable biomaterial. Currently, however, no satisfying long-term vitreous substitute is in clinical use. A novel therapeutic concept represents the combination of hyalocytes with suitable biomaterials. The goal of the present study was to evaluate the potential of bFGF and TGF-beta 1 as tools to control hyalocyte proliferation and the accumulation of extracellular matrix (ECM). Methods In vitro investigation on the influence of different concentrations of bFGF and TGF-beta 1 on hyalocyte morphology, proliferation and ECM production. Results Both growth factors affected hyalocyte morphology; small, round cells could be observed after bFGF supplementation, whereas the cells appeared more completely spread when cultured with TGF-beta 1. Hyalocyte proliferation was increased 3-fold by 10 ng/ml bFGF; 1 ng/ml TGF-beta 1 in contrast reduced cell proliferation to about 40% of the control. Converse effects of the growth factors could also be observed on the ECM accumulation of hyalocytes; whereas bFGF halved ECM accumulation, TGF-beta 1 enhanced the ECM production up to 3-fold. Precultivation of hyalocytes with bFGF for two passages had no influence on their subsequent accumulation of glycosaminoglycans (GAG). However, cells precultivated with bFGF exhibited a doubled accumulation of collagen compared to controls. Conclusions The observed opposite effects of bFGF and TGF-beta 1 on hyalocyte proliferation and ECM accumulation may allow for the control of hyaloycte properties. Therefore, these two growth factors seem to be valuable tools towards the development of a cell-based vitreous substitute.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FIBROBLAST-GROWTH-FACTOR; FACTOR-BETA; TGF-BETA; CELL MIGRATION; VITREOUS GEL; FACTOR FGF; TISSUE; ACID; DIFFERENTIATION; EXPRESSION; hyalocyte; transforming growth factor beta (TGF-beta); basic fibroblast growth factor (bFGF); cell-based vitreous substitute; tissue engineering |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Oct 2020 08:00 |
| Last Modified: | 26 Oct 2020 08:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30372 |
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