Weigert, Johanna and Neumeier, Markus and Bauer, Sabrina and Mages, Wolfgang and Schnitzbauer, Andreas A. and Obed, Aiman and Groeschl, Benedikt and Hartmann, Arndt and Schaeffler, Andreas and Aslanidis, Charalampos and Schoelmerich, Juergen and Buechler, Christa (2008) Small-interference RNA-mediated knock-down of aldehyde oxidase 1 in 3T3-L1 cells impairs adipogenesis and adiponectin release. FEBS LETTERS, 582 (19). pp. 2965-2972. ISSN 0014-5793,
Full text not available from this repository. (Request a copy)Abstract
Aldehyde oxidase 1 (AOX1) is highly abundant in the liver and oxidizes aldehydes thereby generating reactive oxygen species. Enzymes involved in detoxification of aldehydes are expressed in adipocytes and alter adipogenesis, therefore the functional role of AOX1 in adipocytes was analyzed. AOX1 mRNA was higher in visceral compared to subcutaneous human adipose tissue but AOX1 protein was detected in both fat depots. AOX1 expression in adipocytes was confirmed by immunohistochemistry and immunoblot. AOX1 was induced during adipocytic differentiation and was downregulated by fenofibrate in differentiated cells. Knock-down of AOX1 in preadipocytes led to impaired lipid storage and adiponectin release in the differentiated cells. These data indicate that AOX1 is essential for adipogenesis and may link energy and drug metabolism. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACTIVATED-RECEPTOR-GAMMA; VISCERAL ADIPOSE-TISSUE; LIVER ALDEHYDE OXIDASE; OXIDATIVE STRESS; ADIPOCYTE DIFFERENTIATION; GENE-EXPRESSION; POTENTIAL ROLE; MESSENGER-RNA; FATTY-ACIDS; PROTEIN; adipocytes; adipose tissue; peroxisome proliferator-activated receptor agonist |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin Medicine > Lehrstuhl für Pathologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Oct 2020 12:39 |
| Last Modified: | 26 Oct 2020 12:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30463 |
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