Stoegbauer, Fabian and Neumeier, Markus and Weigert, Johanna and Wanninger, Josef and Grandl, Margot and Lehle, Karla and Schmitz, Gerd and Aslanidis, Charalampos and Schaeffler, Andreas and Schoelmerich, Juergen and Buechler, Christa (2008) Highly efficient and low-cost method to isolate human blood monocytes with high purity. JOURNAL OF IMMUNOLOGICAL METHODS, 337 (1). pp. 78-80. ISSN 0022-1759,
Full text not available from this repository. (Request a copy)Abstract
Several techniques are available to purify circulating blood monocytes for research. CD14-containing MicroBeads are suitable and reliable tools to reproducibly isolate human monocytes with a high purity but are quite expensive. This report describes that a comparable number of highly pure monocytes can be isolated from samples using up to tenfold lower amounts of CD14-MicroBeads. MicroBeads are widely used to isolate different cell populations and with this report more researchers may be encouraged to use this highly efficient, low-cost and thus affordable method to pursue their scientific goals. (C) 2008 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXPRESSION; blood monocytes; CD14 MicroBeads; purity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Oct 2020 09:14 |
| Last Modified: | 26 Oct 2020 09:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30465 |
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