Nyhlen, Jonas and Martin-Matute, Belen and Sandstrom, Anders G. and Bocola, Marco and Backvall, Jan-E. (2008) Influence of delta-functional groups on the enantiorecognition of secondary alcohols by Candida antarctica lipase B. CHEMBIOCHEM, 9 (12). pp. 1968-1974. ISSN 1439-4227, 1439-7633
Full text not available from this repository. (Request a copy)Abstract
The selectivity of acetylation of delta-functionalized secondary alcohols catalyzed by Candida antarctica lipase B has been examined by molecular dynamics. The results from the simulation show that a delta-alcohol functionality forms a hydrogen bond with the carbonyl group of Thr 40. This interaction stabilizes the tetrahedral intermediate and thus leads to selective acetylation of the R enantiomer. A stabilizing interaction of the delta-(R)-acetoxy group with the peptide NH of alanine 282 was also observed. No stabilizing interaction could be found for the delta-keto functionality, and it is proposed that this is the reason for the experimentally observed decrease in enantioselectivity. From these results, it was hypothesized that the enantioselectivity could be restored by mutating the alanine in position 281 for serine. The mutation was made experimentally, and the results show that the E value increased from 9 to 120.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CATALYZED TRANSESTERIFICATION REACTIONS; KINETIC ASYMMETRIC TRANSFORMATION; ORGANIC-SOLVENTS; SERINE PROTEASES; CEPACIA LIPASE; AB-INITIO; PSEUDOMONAS-CEPACIA; MOLECULAR-DYNAMICS; CRYSTAL-STRUCTURE; ENANTIOSELECTIVITY; bioorganic chemistry; Candida antarctica lipase B; molecular dynamics; molecular modeling; organic chemistry |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Oct 2020 13:56 |
| Last Modified: | 26 Oct 2020 13:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30483 |
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