Van Blerkom, Jonathan and Davis, Patrick and Thalhammer, Verena (2008) Regulation of mitochondrial polarity in mouse and human oocytes: the influence of cumulus derived nitric oxide. MOLECULAR HUMAN REPRODUCTION, 14 (8). pp. 431-444. ISSN 1360-9947,
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Whether exogenous factors influenced the level of mitochondrial polarity (Delta Psi m) in the subplasmalemmal cytoplasm of the oocyte was investigated with denuded and cumulus-enclosed human and mouse oocytes between the germinal vesicle and metaphase II stage. Co-culture of denuded oocytes with cumulus masses or primary cumulus cell cultures demonstrated a 'proximity' effect with respect to the detectable level of Delta Psi m in the oocyte. The specificity and reversibility of this effect on subplasmalemmal mitochondria were shown by repeated repositioning between cellular and acellular regions, which sequentially down- or up-regulated Delta Psi m. Experimental studies with a nitric oxide (NO) donor and inhibitor of NO synthase indicate that NO produced by cumulus cells has a regulatory influence on Delta Psi m in the subplasmalemmal cytoplasm of the corresponding oocyte. Culture of denuded and cumulus-enclosed (intact) oocytes in low and high oxygen atmospheres suggests that competition between oxygen and NO at the mitochondrial level may regulate the level of Delta Psi m and maintain mitochondria homeostasis in the pre-ovulatory oocyte, with a shift to higher polarity occurring after ovulation. The role of exogenous influences on oocyte Delta Psi m is discussed with respect to the regulation of developmental processes in the oocyte and early embryo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DELTA-PSI-M; IN-VITRO; MEIOTIC MATURATION; PREIMPLANTATION DEVELOPMENT; DEVELOPMENTAL SIGNIFICANCE; MICROTUBULE ORGANIZATION; FOLLICULAR DEVELOPMENT; EMBRYO DEVELOPMENT; ATP CONTENT; RAT OVARY; mitochondria; mitochondrial polarity; nitric oxide; cumulus cells; regulation of mitochondrial activity |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Oct 2020 05:35 |
| Last Modified: | 28 Oct 2020 05:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30552 |
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