Brandhorst, Gunnar and Marquet, Pierre and Shaw, Leslie M. and Liebisch, Gerhard and Schmitz, Gerd and Coffing, Mary Jane and Domke, Ingrid and Streit, Frank and Luthe, Hilmar and Oellerich, Michael (2008) Multicenter evaluation of a new inosine monophosphate dehydrogenase inhibition assay for quantification of total mycophenolic acid in plasma. THERAPEUTIC DRUG MONITORING, 30 (4). pp. 428-433. ISSN 0163-4356,
Full text not available from this repository. (Request a copy)Abstract
The performance characteristics of a new inosine monophosphate dehydrogenase inhibition assay for the quantification of total mycophenolic acid (MPA) in plasma (Roche Diagnostics) were assessed in a multicenter evaluation. Validation data were collected from four institutions. Within-run and total imprecision were acceptable (n = 21 for each of 7 materials, coefficients of variation ranging 0.7-9.6%). The lower limit of quantification was 0.31 mg/L. The assay was linear from 0.31 to 15.0 mg/L. Method comparison with validated high-performance liquid chromatography with ultraviolet light or liquid chromatography tandem mass spectrometry methods showed good agreement (coefficients of correlation 0.974-0.994, slopes 1.01-1.17, intercepts -0.17 to 0.06). There was no difference found between results from different transplant types (cardiac vs. renal) or comedications (cyclosporine vs. tacrolimus). The recovery of samples from a proficiency testing scheme was acceptable. The cross-reactivity of AcMPAG, an in vitro active metabolite of MPA, was examined by adding AcMPAG to a pool of patient samples and subsequent quantification. MPA overestimation by AcMPAG cross-reactivity was found to be low (< 5%). Thus, this interference is expected to be clinically irrelevant. In conclusion, the Roche Total MPA assay is a promising alternative for MPA quantification where chromatographic methods are not available.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; RENAL-TRANSPLANT RECIPIENTS; ENZYME-MULTIPLIED IMMUNOASSAY; ACYL GLUCURONIDE METABOLITE; MOFETIL; EMIT; mycophenolic acid; mycophenolate mofetil; MPA; therapeutic drug monitoring; inosine monophosphate dehydrogenase |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Oct 2020 06:51 |
| Last Modified: | 28 Oct 2020 06:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/30572 |
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